Plasmid Curation

From DictyWiki

Plasmids are divided into the following types:

• natural plasmids
• vectors
• constructs
  • overexpression
  • knockouts

• Plasmids names should always start with small case p

Contents 1 Natural Plasmids 2 Vectors 3 Overexpression/fusion constructs 4 Knock-out constructs

Natural Plasmids

Vectors

Overexpression/fusion constructs

Examples:

• pDneo67/act6:regA
• if the vector is not available: Use the gene name directly:
  • pTagB
  • pAct15-GFP

Knock-out constructs

Examples:

• pBSR1-sadA-KO
• pA15-BSR/sadA-KO

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