Plasmid Curation

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Plasmids are divided into the following types:

  • natural plasmids
  • vectors
  • constructs
    • overexpression
    • knockouts


  • Plasmids names should always start with small case p

Contents

Natural Plasmids

Vectors

Overexpression/fusion constructs

Examples:

  • pDneo67/act6:regA
  • if the vector is not available: Use the gene name directly:
    • pTagB
    • pAct15-GFP

Knock-out constructs

Examples:

  • pBSR1-sadA-KO
  • pA15-BSR/sadA-KO



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