Vectors

From DictyWiki

Jump to: navigation, search

This page is a summary of Dicty cloning vectors.

Vector Name
Promoter || Fusion || Drug Resistance || Extrachr / Integrative (ORI) || PMID || GenBank Acc



Contents

pA15DBamGAL

act15prom || gene - lacZ || neoR || Integrative (no Dicty ORI?) || PMID 2198542

  • Compared to pDdlac-1 (Dingermann et al 1989), removes fragment of that caused instability of the plasmid
  • Removes most of the act15 promoter, keeping the ATG and other basal expression elements


pA15GFP

act15prom ||gene - GFP || neoR || Integrative || PMID 7489901 || GenBankAcc

  • C-terminal GFP fusion


pA15LUC

Promoter: None || Luciferase(act15P/act15T) || neoR || Integrative? (no ORI?) || Lloyd et al 1990 Dev Genet 11:391 || GenBankAcc

p1ABsr8

act15prom || gene - GFP || bsR || Extrachr (Ddp2 ORI) || PMID 10769206 || GenBank Acc

  • Derived from pB15-GFP where the SacI site has been inactivated (pB15GFPXSac)
  • neoR has been replaced with bsR
  • C-terminal GFP fusion vector


pATANB43

Promoter: None || Fusion: None || neoR (act6P) || Extrachromosomal (Ddp1) || PMID 2813371 || GenBank Acc

pB10TP1

Promoter || Fusion || neoR || Extrachr / Integrative (ORI) || PMID 3436528 || GenBank Acc

pB10TP2

Promoter || Fusion || neoR || Extrachr / Integrative (ORI) || PMID 3436528 || GenBank Acc

pB15-GFP

act15prom || gene - GFP || neoR || Extrachr (Ddp2 ORI) || PMID 10769206 || GenBank Acc

  • Derived from pDXA-3C
  • C-terminal GFP fusion vector


pB17S

act15 || gene-HA || neoR || Extrachr / Integrative (ORI) || PMID 15851518 || GenBank Acc

  • Not original reference. Authors cite PMID 7557400 but this does not mention pB17S.


pBIG

act15 promoter || Fusion: None || neoR (act6P) || Extrachr (Ddp1 ORI) || PMID 7691416 || GenBank Acc
Notes:

  • vector sequence from pBluescript II SK


pBORB

act15 promoter || Fusion: None || neoR (act6P) || Integrative || PMID ? || GenBank Acc
Notes:

  • created from memory in Rex's lab, came across in PMID 8816999 where it's not much described except neoR.


pBSrH

act15 promoter || Fusion: None || bsR (act15P) || Integrative || PMID 11352578 || GenBank Acc
Notes:

  • Not much described in PMID 11352578, but map is in Stock Center, plasmid 414.


pCFC5

act15 promoter || Fusion: N-terminal T7-tag || neoR || Integrative|| PMID 9668968 || GenBank Acc
Notes:

  • vector sequence from pEXP4(+)


pDdGal15

act15prom || gene - lacZ || neoR || Integrative (no Dicty ORI?) || PMID 2198542

  • Compared to pDdlac-1 (Dingermann et al 1989), removes fragment of that caused instability of the plasmid


pDdGal16

act15prom || gene - lacZ || neoR || Integrative (no Dicty ORI?) || PMID 2198542

  • Compared to pDdlac-1, (Dingermann et al 1989) removes fragment of that caused instability of the plasmid


pDdGal17

act15prom || gene - lacZ || neoR || Integrative (no Dicty ORI?) || PMID 2198542

  • Compared to pDdlac-1, (Dingermann et al 1989) removes fragment of that caused instability of the plasmid


pDE104

Integrative vector with hygromycin selection
Promoter || Fusion || Hygromycin (act15P/act15T) || Integrative (no Dicty ORI) || PMID 25460565 || GenBank Acc
Notes: Hygromycin selection does always not works reproducibly

pDE109

Extrachromosomal vector with hygromycin selection
Promoter || Fusion || Hygromycin (act15P/act15T) || Extrachr (Ddp2 ORI) || PMID 25460565 || GenBank Acc

pDEX H

[act15] || GFP || [act6]:neoR || Integrative || PMID 1328260 || GenBank Acc
Notes: based on the picture in PMID 1328260, there is no Dp1 or Dp2 Ori, so it is integrative (1-6-09 PF)
Notes: based on pIC20R; from PMID 7954798 I believe the pDEX plasmids are integrative (PF)

pDGFP-MCS-Neo

[act15] || GFP || [act6]:neoR || Integrative || PMID 10825297 || GenBank Acc
Notes: A 700 bp sequence encoding the red-shifted GFP S65T (Heim and Tsien, 1996) together with an in-frame sequence encoding the linker (GGS)2 and a multiple cloning site containing SalI, HincII, XhoI and EcoRI restriction sites behind the GFP sequence was excised with HindIII from vector pDGFP-MCS and inserted in sense orientation in the expression vector pDEXH (Faix et al., 1992). The resulting vector was termed pDGFP-MCS-Neo. From PMID 10825297

pDIV1

3.8 kb spanning the pyr5-6 gene cloned into pGEM3 (Promega)|| Integrative (ORI) || PMID 1326764|| GenBank Acc
Notes: integrative plasmid with the pyr5-6 selection marker that selects for ura+ prototrophy

pDIV2

3.8 kb spanning the pyr5-6 gene cloned into pGEM3 (Promega)|| Integrative (ORI) || PMID 1326764|| GenBank Acc
Notes: integrative plasmid with the pyr5-6 selection marker that selects for ura+ prototrophy - the pyr5-6 gene is in opposite direction as compared to pDIV1

pDJS

[dscB](discoidin) || Fusion || neoR || Extrachr / Integrative (ORI) || PMID 15289548 (not the primary reference) || GenBank Acc

pDM131

[act15] || mRFPmars:gene || no drug resistance || Extrachr (Ddp1-based) || PMID 19063918 || EU912545

pDM193

[act15] || GST:thrombin protease recognition sequence:gene || no drug resistance || Extrachr (Ddp1-based) || PMID 19063918 || EU912549

pDM229

[act15] || TAP-Tobacco Etch Virus protease recognition sequence:calmodulin binding peptide:gene || no drug resistance || Extrachr (Ddp1-based) || PMID 19063918 || EU912547

pDM276

[act15] || gene:TAP || no drug resistance || Extrachr (Ddp1-based) || PMID 19063918 || EU912548

pDM304

[act15] || No fusion || neoR || Extrachr (Ddp1-based) || PMID 19063918 || EU912539
Note: Modular expression vector : Shuttle vector used to express a second gene on the modular expression vector pDM344.

pDM309

TRE-Pmin (7 repeats of the TetO operator fused to a minimal fragment of the act15 promoter) || No fusion || tTA transactivator || neoR || Extrachr (Ddp1-based) || PMID 19046986 ||EU908848

pDM310

TRE-Pmin (7 repeats of the TetO operator fused to a minimal fragment of the act15 promoter) || No fusion || tTA-M2 transactivator || neoR || Extrachr (Ddp1-based)|| PMID 19046986 ||EU908845

pDM312

[act15] || gene:mRFPmars || no drug resistance || Extrachr (Ddp1-based) || PMID 19063918 || EU912546

pDM313

[act15] || gene:GFP || no drug resistance || Extrachr (Ddp1-based) || PMID 19063918 || EU912544

pDM314

[act15] || GST:gene || neoR || Extrachr (Ddp1-based) || PMID 19063918

pDM314

[act15] || TAP:gene || neoR || Extrachr (Ddp1-based) || PMID 19063918


pDM317

[act15] || GFP:gene || neoR || Extrachr (Ddp1-based) || PMID 19063918

pDM318

[act15] || mRFPmars:gene || neoR || Extrachr (Ddp1-based) || PMID 19063918

pDM320

[act15] || FLAG:gene || neoR || Extrachr (Ddp1-based) || PMID 19063918

pDM321

[act15] || gene:TAP || neoR || Extrachr (Ddp1-based) || PMID 19063918

pDM323

[act15] || gene:GFP || neoR || Extrachr (Ddp1-based) || PMID 19063918

pDM324

[act15] || gene:mRFPmars || neoR || Extrachr (Ddp1-based) || PMID 19063918

pDM326

[act15] || No fusion || bsrR || Extrachr (Ddp1-based) || PMID 19063918 || EU912541
Note: Modular expression vector : Shuttle vector used to express a second gene on the modular expression vector pDM344.

pDM327

Shuttle vector ||[act15] || GFP: gene || no drug resistance || PMID 19063918

pDM328

Shuttle vector ||[act15] || mRFPmars: gene || no drug resistance || PMID 19063918

pDM329

Shuttle vector ||[act15] || gene:GFP || no drug resistance || PMID 19063918

pDM330

Shuttle vector ||[act15] || gene:mRFPmars || no drug resistance || PMID 19063918

pDM331

TRE-Pmin (7 repeats of the TetO operator fused to a minimal fragment of the act15 promoter) || GST-gene || tTA-M2 transactivator || neoR || Extrachr (Ddp1-based)|| PMID 19046986

pDM332

TRE-Pmin (7 repeats of the TetO operator fused to a minimal fragment of the act15 promoter) || TAP-gene || tTA-M2 transactivator || neoR || Extrachr (Ddp1-based)|| PMID 19046986

pDM334

TRE-Pmin (7 repeats of the TetO operator fused to a minimal fragment of the act15 promoter) || GFP-gene || tTA-M2 transactivator || neoR || Extrachr (Ddp1-based)|| PMID 19046986

pDM335

TRE-Pmin (7 repeats of the TetO operator fused to a minimal fragment of the act15 promoter) || mRFPmars-gene || tTA-M2 transactivator || neoR || Extrachr (Ddp1-based)|| PMID 19046986

pDM338

TRE-Pmin (7 repeats of the TetO operator fused to a minimal fragment of the act15 promoter) || gene-TAP || tTA-M2 transactivator || neoR || Extrachr (Ddp1-based)|| PMID 19046986

pDM340

TRE-Pmin (7 repeats of the TetO operator fused to a minimal fragment of the act15 promoter) || gene-GFP || tTA-M2 transactivator || neoR || Extrachr (Ddp1-based)|| PMID 19046986

pDM341

TRE-Pmin (7 repeats of the TetO operator fused to a minimal fragment of the act15 promoter) || gene-mRFPmars || tTA-M2 transactivator || neoR || Extrachr (Ddp1-based)|| PMID 19046986

pDM344

Shuttle vector NgoMIV || [act15] || no drug resistance || PMID 19063918 ||FJ402941

pDM347

Gateway conversion cassette || PMID 19063918 || EU912550


pDM351

[act15] || GFP:gene || neoR || gateway MCS || Extrachr (Ddp1-based) || PMID 19063918

pDM352

[act15] || mRFPmars:gene || neoR || gateway MCS || Extrachr (Ddp1-based) || PMID 19063918

pDM353

[act15] || gene:GFP || neoR || gateway MCS || Extrachr (Ddp1-based) || PMID 19063918

pDM354

[act15] || gene:mRFPmars || neoR || gateway MCS || Extrachr (Ddp1-based) || PMID 19063918

pDM358

[act15] || No fusion || hygR || Extrachr (Ddp1-based) || PMID 19063918 || EU912542
Note: Modular expression vector : Shuttle vector used to express a second gene on the modular expression vector pDM344.

pDM359

TRE-Pmin (7 repeats of the TetO operator fused to a minimal fragment of the act15 promoter) || No fusion || tTA-M2 transactivator || hygR || Extrachr (Ddp1-based)|| PMID 19046986 ||EU908846

pDM360

TRE-Pmin (7 repeats of the TetO operator fused to a minimal fragment of the act15 promoter) || No fusion || tTA-M2 transactivator || bsrR || Extrachr (Ddp1-based)|| PMID 19046986 ||EU908847

pDM369

TRE-Pmin (7 repeats of the TetO operator fused to a minimal fragment of the act15 promoter) || GFP-gene || tTA-M2 transactivator || hygR || Extrachr (Ddp1-based)|| PMID 19046986

pDM370

TRE-Pmin (7 repeats of the TetO operator fused to a minimal fragment of the act15 promoter) || gene-GFP || tTA-M2 transactivator || hygR || Extrachr (Ddp1-based)|| PMID 19046986

pDM371

TRE-Pmin (7 repeats of the TetO operator fused to a minimal fragment of the act15 promoter) || gene-GFP || tTA-M2 transactivator || hygR || Extrachr (Ddp1-based)|| PMID 19046986
Note: same as pM369, but with Gateway MCS (pM369 has BglII/SpeI MCS)

pDM372

TRE-Pmin (7 repeats of the TetO operator fused to a minimal fragment of the act15 promoter) || GFP-gene || tTA-M2 transactivator || hygR || Extrachr (Ddp1-based)|| PMID 19046986
Note: same as pM370, but with Gateway MCS (pM370 has BglII/SpeI MCS)

pDM373

TRE-Pmin (7 repeats of the TetO operator fused to a minimal fragment of the act15 promoter) || No fusion || tTA-M2 transactivator || hygR || Extrachr (Ddp1-based)|| PMID 19046986
Note: same as pM359, but with Gateway MCS (pM359 has BglII/SpeI MCS)

pDM410

Shuttle vector ||[act15] || GFP: gene || no drug resistance || gateway MCS || PMID 19063918

pDM411

Shuttle vector ||[act15] || mRFPmars: gene || no drug resistance || gateway MCS || PMID 19063918

pDM412

Shuttle vector ||[act15] || gene:GFP || no drug resistance || gateway MCS || PMID 19063918

pDM413

Shuttle vector ||[act15] || gene:mRFPmars || no drug resistance || gateway MCS || PMID 19063918


pDM414

Shuttle vector ||[act15] || no fusion|| no drug resistance || gateway MCS || PMID 19063918

pDM448

[act15] || GFP:gene || hygR || gateway MCS || Extrachr (Ddp1-based) || PMID 19063918

pDM449

[act15] || mRFPmars:gene || hygR || gateway MCS || Extrachr (Ddp1-based) || PMID 19063918

pDM450

[act15] || gene:GFP || hygR || gateway MCS || Extrachr (Ddp1-based) || PMID 19063918

pDM451

[act15] || gene:mRFPmars || hygR || gateway MCS || Extrachr (Ddp1-based) || PMID 19063918

pDNeoII

act6prom || no fusion || neoR || Integrative || PMID 2832153

pDneo2a-GFP

act6prom || gene:GFP || neoR || Integrative || PMID 20600701

pDRH

act15prom || no fusion || hygR || Extrachr (Ddp1-based) || PMID 17050732 [1]

pDV-NTAP

[act15] || gene -GFP || neoR || Integrative || PMID 17296313 || GenBank [2] EF028665 Notes: Part of a series of TAP-tagged +/- GFP vectors (see pDV series page)

pDV-CGFP

[act15] || gene -GFP || neoR || Integrative || PMID 17296313 || GenBank [3] EF028667 Notes: Part of a series of TAP-tagged +/- GFP vectors (see pDV series page)

pDXA-3C

act15prom || gene - myc || neoR || Extrachr (Ddp2 ORI) || PMID 7557400 || GenBank X85118

pDXA-3D

act15 || gene - myc || neoR || Extrachr (Ddp2 ORI) || PMID 14551196 || GenBank
Notes: Altered pDXA-3C; ATG upstream of the cDNA insertion site removed

pDXA-3H

act15prom || gene - 8xHis || neoR || Extrachr (Ddp2 ORI) || PMID 7557400 || GenBank X85119

pDXA-FLAG

act15prom || FLAG - gene - myc || neoR || Extrachr (Ddp2 ORI) || PMID 11078649
Notes:
C terminal myc tag not translated if the cDNA provides its own stop codon.

pDXA-GFP

act15prom || 7xHis-GFP fusion - myc || neoR || Extrachr (Ddp1 ORI) || PMID 11078649
Notes:

  • GFP fusion is at C terminus if KpnI is used; at N terminus if SacI, BamHI, XhoI are used.
  • 8xHis tag always N terminal.
  • GFP's C terminal myc tag is not recogized by 9E10 antibody.


pDXA-GFP2

act15prom || 8xHis - GFP fusion- myc || neoR || Extrachr (Ddp2 ORI) || PMID 11078649
Notes:

  • GFP fusion is at C terminus if KpnI is used; at N terminus if SacI, BamHI, XhoI are used.
  • 8xHis tag always N terminal.
  • GFP's C terminal myc tag is not recogized by 9E10 antibody.


pDXA-HC

act15prom || 7xHis - gene - myc || neoR || Extrachr (Ddp2 ORI) || PMID 7557400 || GenBank X85123 Notes: C terminal myc tag not translated if the cDNA provides its own stop codon.

pDXA-HY

act15prom || 8xHis - gene - YL1/2 || neoR || Extrachr (Ddp2 ORI) || PMID 7557400 || GenBank X85122

pDXD-3C

dscBprom || gene - myc || neoR || Extrachr (Ddp2 ORI) || PMID 7557400 || GenBank X85121

pDXD-3H

dscBprom || gene - 8xHis || neoR || Extrachr (Ddp2 ORI) || PMID 7557400 || GenBank X85120

pEXP4(+)

act15 || Fusion || neoR || Integrative || PMID 7926779, PMID 17296313 || GenBank EF028663
Notes:

  • Based on commercial vector pAT153 (see PMID 17296313)
  • pAT153 leads to pATPS, leads via pEXP1, 2, 3 to EXP4(+) (PMID 7926779)


pEXP5(+)

act15 || Fusion || neoR || Integrative || PMID 17296313 || GenBank EF028664
Notes:


pEZTN:tetR-bsR

Promoter || Fusion || [act15]:BSR || Integrative (ORI) || PMID 12954783 || GenBank Acc Notes: Generates a transposable blasticidin resistance (bsr) cassette

pfeI

Promoter: None || Fusion: None || Bleomycin (phleomycin)(act15P/act8T) || Extrachr (Ddp2 ORI) || PMID 1953786 || GenBank Acc

pfeII

Promoter: None || Fusion: None || Bleomycin (phleomycin)(act15P/act8T) || Extrachr (Ddp2 ORI) || PMID 1953786 || GenBank Acc

pJK1

Promoter: act15; 2H3 terminator || Fusion: None || neoR (act6P) || Extrachrom || Ddp1 || PMID 1348970 || GenBank Acc
Notes

  • Based on pATANB43


pLittle

act15 promoter || Fusion: None || neoR (act6P) || Extrachr (Ddp1 ORI) || PMID 8567716 || GenBank Acc
Notes:

  • derivative of pBIG
  • differs from pBIG in that a 1.5-kb KpnI fragment in the Ddpl portion of pBIG was removed.


pLD1A15SN

act15 promoter || Fusion: None || neoR (act6P) || Extrachr (Ddp2 ORI) || PMID 10953006 || GenBank Acc
Notes:

  • expression plasmid used for suppressor screen


pLPBLP

act15 promoter || Fusion || bsR || loxP sites || Integrative || PMID 15507682 || GenBank Acc

pMB35

act15 promoter || Fusion: transactivator (tTAs*) || neoR, tetR || Integrative || PMID 10903439 || GenBank Acc
Notes:

  • transactivator plasmid, Tet on/off system


pMB38

inducible promoter TRE-Pmin || Fusion: None || bsR, || Extrachr (ORI?) || PMID 10903439 || GenBank Acc
Notes:

  • response plasmid
  • bsR under control of act15 minimal promoter
  • Tet on/off system: inducible promoter (TRE-Pmin) upstream of the D. discoideum 2H3T terminator (2H3T)


pMB74

act15 promoter || Fusion: might have GFP (MB74-GFP) || neoR, || Extrachr (ORI?) || PMID 15827084, 17040207|| GenBank Acc
Notes:



pMUW161

Bleomycin casette with the act6 promoter and the act8 terminator
Promoter: None || Fusion: None || Bleomycin (act6P/act8T) || Extrachr (Ddp2 ORI) || PMID 1720754 || GenBank Acc


pnDEL

act15 promoter || Fusion: None || neoR (act6P)? || Integrative || PMID 3244804 || GenBank Acc

pPAV

act15 promoter || Fusion: CAT:act15T || Drug resistance: none (must be co-transformed) || Extrachr (Ddp2 ORI) || PMID 2825142 || GenBank Acc

pPT130

act15prom || no fusion || neoR || Extrachr / Integrative (ORI) || PMID 16765443
Notes: No Kozak or ATG.

pREP

Plasmid that confers the Ddp2 ORI trans replication functions || PMID 11078649 (This is not the primary reference)

pTIKL

act15 promoter || Fusion: None || neoR (act6P) || Extrachr (Ddp1 ORI) || PMID 12135375 ||


pTS1

Promoter: None || Fusion: None || neoR/Thymidylate Synthase+ || Extrachr / Integrative (ORI) || PMID 2734099 || GenBank Acc

pTX-FLAG

act15prom || FLAG - epitope || neoR || Extrachr (Ddp1 ORI) || PMID 11078649
Notes:

  • If the cDNA provides its own stop codon the epitope will not be present in the expressed protein.
  • Ddp1 ORI confers more uniform expression across individual cells.


pTX-GFP

act15prom || 8xHis - GFP fusion - myc || neoR || Extrachr, (Ddp1 ORI) || PMID 11078649
Notes:

  • GFP fusion is at C terminus if KpnI is used; at N terminus if SacI, BamHI, XhoI are used.
  • 8xHis tag always N terminal.
  • GFP's C terminal myc tag is not recogized by 9E10 antibody.
  • Ddp1 ORI confers more uniform expression across individual cells.


pV18-Tn5

Can be used to transfom non-axenic strains
Promoter || Fusion || noeR(V18P/P8A7T) || Extrachr / Integrative (ORI) || PMID 9007012 || GenBank Acc

pVEII

For repressible expression; folate-inducible
dscBprom (discoidin gamma) || no fusion || neoR || Integrative (ORI) || PMID 1475184 || GenBank Acc

pYU20

act15prom neoR || Extrachr (ORI ?) || PMID 17664333


[Back to Internal Documentation]

Personal tools